| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141409 |
| Chromosome: | chromosome 10 |
| Location: | 4330060 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451000 | TRR1 | (1 of 1) K15407 - queuine tRNA-ribosyltransferase subunit QTRTD1 [EC:2.4.2.29] (QTRTD1); Queuine tRNA-ribosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTCACGACACAGGCGGTGAAGGGACGA |
| Internal bar code: | AGGCTGCGTGCGACGTGCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 99.8988 |
| LEAP-Seq n confirming: | 987 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGTCAGGTAGGGATGC |
| Suggested primer 2: | ACCTGCTCACCTCTCAGCAT |