| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141416 |
| Chromosome: | chromosome 9 |
| Location: | 7050520 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411400 | MOT45 | PF13 protein required for dynein inner/outer arm assembly in the | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACAGAGACAGTCCACTGGCGAGCGTG |
| Internal bar code: | CACAGACATCGTGGACTTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 99.8646 |
| LEAP-Seq n confirming: | 7375 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTTGGAAAGAAGGTCTG |
| Suggested primer 2: | TTACGCACACTGCACACGTA |