Insertion junction: LMJ.RY0402.141416_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141416
Chromosome:chromosome 17
Location:116526
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g697000 (1 of 2) PTHR23155:SF401 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAATCTGGGTCACCCGATGCACCCGCAC
Internal bar code:AGTTCGCACAGACGCACGAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:639
LEAP-Seq percent confirming:88.5629
LEAP-Seq n confirming:12738
LEAP-Seq n nonconfirming:1645
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGAAGGTTTGTCCGTGG
Suggested primer 2:GACATACAGCAGTCTGGCGA