| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141435 |
| Chromosome: | chromosome 10 |
| Location: | 5099895 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456150 | HLD3 | (1 of 1) PTHR10992:SF701 - SERINE HYDROLASE-RELATED; Haloalkane dehalogenase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGTGTACTGGGCGCGTGTCCACCTAT |
| Internal bar code: | CTCTGATACCGAGGCGGAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 88.6184 |
| LEAP-Seq n confirming: | 1347 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAGTGTGCGAGAGTGGC |
| Suggested primer 2: | AAACGATCGGTTACAAACGC |