| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141496 |
| Chromosome: | chromosome 3 |
| Location: | 7467269 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205700 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTAGTATGACACTTAGGGGGAGCGCTTT |
| Internal bar code: | CCCTGAATCTCTTCACTGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 70.084 |
| LEAP-Seq n confirming: | 2003 |
| LEAP-Seq n nonconfirming: | 855 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAACAGCACCTCGTGAAG |
| Suggested primer 2: | GGCAGCTGAGACGGAACTAC |