Insertion junction: LMJ.RY0402.141510_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.141510
Chromosome:chromosome 7
Location:5413254
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g350300 (1 of 31) IPR016187 - C-type lectin fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGTTGTGGGTGCGTGGGTGTTCGTCT
Internal bar code:GTTTCATCTGTTCATACAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1358
LEAP-Seq percent confirming:99.8542
LEAP-Seq n confirming:1370
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGCGTGGTTATGACAAT
Suggested primer 2:ACGTACGGAGCTTGTTTGCT