Insertion junction: LMJ.RY0402.141520_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141520
Chromosome:chromosome 3
Location:3329250
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g166400 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCACGTGAGGTTCGCCGGCGAGCGGCG
Internal bar code:GGACATTAGGAACCAACGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:98.8422
LEAP-Seq n confirming:1622
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACGGGTAGACCTTCTTG
Suggested primer 2:CCAAAAGCACGATTCCATTT