Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.141590 |
Chromosome: | chromosome_9 |
Location: | 2140624 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393450 | FAP233 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCTGGATGTAGCCCTTGGGCGCGTCGTAGG |
Internal bar code: | GGCGCGGGTAACGTAGGTGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 694 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 801 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCTGGTCATGTGGACGTA |
Suggested primer 2: | GTGGCCATAGTGTGATGTGC |