| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141614 |
| Chromosome: | chromosome_2 |
| Location: | 750638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g078550 | FAP210 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTGCGTAGCTATCATACAAATTCGGGCC |
| Internal bar code: | ATTAGCTCTACTCGGTATTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.6926 |
| LEAP-Seq n confirming: | 2919 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAGTGCATCGAGGAGTA |
| Suggested primer 2: | ACAAGAAGCGAGGTGGAAGA |