| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141627 |
| Chromosome: | chromosome 3 |
| Location: | 3737907 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169750 | FAP371 | Flagellar Associated Protein 371 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCACGCCCTCCACAGCGTGACAGTGCA |
| Internal bar code: | ACTATCTCGGTGCTTGAGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.6449 |
| LEAP-Seq n confirming: | 3929 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTTGATGATGGGACTT |
| Suggested primer 2: | CATATACCTGCACACCGTCG |