Insertion junction: LMJ.RY0402.141678_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.141678
Chromosome:chromosome 3
Location:7346011
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g206550 CPLD2,PGP5 (1 of 2) PTHR18901//PTHR18901:SF9 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED; conserved expressed protein with hydrolase motif gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGGTCGCGCACACCTCGTCCGAGAGG
Internal bar code:TCGGATGCATAAGGACGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:334
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGGGCTTCCAACCTACT
Suggested primer 2:GGGCTTGAAGAGAGGGTTCT