Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.141683 |
Chromosome: | chromosome_6 |
Location: | 7279961 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g298500 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACACATCATGTAACCCCGGTGCTCCGGAG |
Internal bar code: | ACGAAGTGGCGCGGCGAGGACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 180 |
LEAP-Seq percent confirming: | 99.7177 |
LEAP-Seq n confirming: | 1413 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTGGCAGAGACATTCATT |
Suggested primer 2: | ACAGCTGATGCCGTACACAG |