| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141689 |
| Chromosome: | chromosome 14 |
| Location: | 761570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612850 | (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCACATGCCCGCCTTACTACACCGTGC |
| Internal bar code: | ACCAATTTGTCATCTCATCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 72.7746 |
| LEAP-Seq n confirming: | 1259 |
| LEAP-Seq n nonconfirming: | 471 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTCTGCGGGGAATAGTA |
| Suggested primer 2: | GCGAAGACGATGAAGAGGAC |