Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.141693 |
Chromosome: | chromosome_5 |
Location: | 2132478 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234640 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCGCAACATGGTGCGTGGGGGCGTGGATG |
Internal bar code: | GCTGGTGTTCCCGCTCGGTTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 129 |
LEAP-Seq percent confirming: | 99.8397 |
LEAP-Seq n confirming: | 3115 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGACCATGGCAGTGACAG |
Suggested primer 2: | GCCACGAGGTAAACCAAAAA |