| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141699 |
| Chromosome: | chromosome 6 |
| Location: | 3288823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277100 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACTCTTTGTCGAATGTGCCTTTTTGTG |
| Internal bar code: | ATGAGCCGCACTATGTGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 29.5989 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 1018 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATTCCGCGTCATCATACC |
| Suggested primer 2: | CAAGTCCTAACCACGCCATT |