| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141705 |
| Chromosome: | chromosome 6 |
| Location: | 979675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255750 | EZY1 | Early zygote expressed chloroplast nucleoid localized protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCTCCTCACGAGCCATCTTGACCACG |
| Internal bar code: | TGTCCCTGTTTGCGTGGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 94.186 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTAGTCTCCCGGACGCAC |
| Suggested primer 2: | GGTAGTCCTTGATGGCGTGT |