| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141735 |
| Chromosome: | chromosome 7 |
| Location: | 1203403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321100 | (1 of 1) PTHR10196:SF67 - SEDOHEPTULOKINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATACAAGGACCGGTTGCATGAATGCAGG |
| Internal bar code: | CACGGTAGCTTCACGAGTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.6587 |
| LEAP-Seq n confirming: | 1460 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGTCCGCACATACAGT |
| Suggested primer 2: | GACAATTGCATGTGCGTACC |