Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.141739 |
Chromosome: | chromosome_2 |
Location: | 2809408 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAGCACTCAAAAGCTTTAATTAGAAGTGTT |
Internal bar code: | GGGCGCCCTTTGTTCAGGAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 870 |
LEAP-Seq percent confirming: | 99.9364 |
LEAP-Seq n confirming: | 1572 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGCTTGTAGGAGAGACCTG |
Suggested primer 2: | AACAGGGGAACCTACATCCC |