| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141758 |
| Chromosome: | chromosome 11 |
| Location: | 2463512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475550 | UMM10 | (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCCCCGCGCCACGCACCTGCCGTTTAG |
| Internal bar code: | CGCGTGCAATATTTGTCGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.8886 |
| LEAP-Seq n confirming: | 897 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTTTAGCTACGGAAGCG |
| Suggested primer 2: | GTTGTGACCGGCGCTATTAT |