| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.141783 |
| Chromosome: | chromosome 16 |
| Location: | 127007 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695300 | (1 of 1) K12878 - THO complex subunit 1 (THOC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTGATGACAGCTGCCCGTACATACCC |
| Internal bar code: | GTAGTTTTTTGAAATCATTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 99.7978 |
| LEAP-Seq n confirming: | 5430 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCACCTCCTGAGTTC |
| Suggested primer 2: | GTTTGAAGCTCCGAAAGCAC |