Insertion junction: LMJ.RY0402.141788_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.141788
Chromosome:chromosome 2
Location:3241685
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGGACGGAAGAGGACCCCTTCATAGTT
Internal bar code:GTTAGAGCGTCCATCCACCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:312
LEAP-Seq percent confirming:85.8696
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCAACAACACCAACAGC
Suggested primer 2:CGAATGTATGGGGGTATTGC