| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141801 |
| Chromosome: | chromosome 10 |
| Location: | 2741524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438450 | LTH1 | LAT2 homolog | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCATGGACCAAACCGGCCTACGGCACA |
| Internal bar code: | AGGTCTGGTTGGTCGATATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 99.823 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCGACTTGGTCTTGTTG |
| Suggested primer 2: | CGACGGCCATAATTACTCGT |