| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141817 |
| Chromosome: | chromosome 8 |
| Location: | 825391 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360850 | (1 of 1) K18798 - protein AFG1 (AFG1, LACE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTACCGCTTTCTTCAACCACCTTTGCA |
| Internal bar code: | AGGGAGACAGGCATTTTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 99.869 |
| LEAP-Seq n confirming: | 1525 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACCATCGCATACA |
| Suggested primer 2: | TGGAGGTGTGTGTGTGTGTG |