| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141817 |
| Chromosome: | chromosome_8 |
| Location: | 825391 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g360850 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCACTGAAGGGGTGGAGGGTAGGAGGGA |
| Internal bar code: | TGTCTAAATCAATTCTAGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 94.033 |
| LEAP-Seq n confirming: | 17445 |
| LEAP-Seq n nonconfirming: | 1107 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACCATCGCATACA |
| Suggested primer 2: | TGGAGGTGTGTGTGTGTGTG |