Insertion junction: LMJ.RY0402.141820_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141820
Chromosome:chromosome 7
Location:245513
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g313850 (1 of 1) PTHR22844//PTHR22844:SF219 - F-BOX AND WD40 DOMAIN PROTEIN // WD REPEAT-CONTAINING PROTEIN 49 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCACTCGCTCCTCGGCGTCGGTGATGG
Internal bar code:CTTGCGCGGGTCGGGCACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:436
LEAP-Seq percent confirming:48.381
LEAP-Seq n confirming:762
LEAP-Seq n nonconfirming:813
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTGGTGGTTGTTGTTG
Suggested primer 2:ACACACGCACACACACACAC