| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141867 |
| Chromosome: | chromosome 9 |
| Location: | 4612823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396512 | OPR12 | OctotricoPeptide Repeat protein 12 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGGCAACCGCACCGGCGGATCAACACT |
| Internal bar code: | GTAGGTAACGAGGGTAGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.8077 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACATACGGTGCGTGAAT |
| Suggested primer 2: | AAGGTAGACCATGCCCTGTG |