| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.141868 |
| Chromosome: | chromosome 13 |
| Location: | 558123 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565321 | (1 of 2) 4.3.2.2 - Adenylosuccinate lyase / Succino AMP-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGCGACACCGCTAGCGACGACCTGCAA |
| Internal bar code: | TGCGTCCGGCGAGGACTGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 201 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGTGAGGCGAGGAGCAT |
| Suggested primer 2: | CTGCTTGCCCCTCTCACTAC |