| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.141916 |
| Chromosome: | chromosome 6 |
| Location: | 3306115 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277200 | BIOA,BIO1 | Diaminopelargonic acid synthase; (1 of 1) 6.3.3.3 - Dethiobiotin synthase / DTB synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGACCGAACGGTGGCGCATAGGCGCATT |
| Internal bar code: | AACTGGGCACCTAATTTTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 99.6691 |
| LEAP-Seq n confirming: | 10241 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGAGACCTCGTGGGAT |
| Suggested primer 2: | TGTGATTGGGCGGTAGTGTA |