Insertion junction: LMJ.RY0402.141933_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.141933
Chromosome:chromosome 13
Location:872703
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g567500 (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGCAGCGTTGCCGCGCGCTCCGCCCAC
Internal bar code:TTTTCTGGTCGCAGTTTAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAACAACATCAGCAGCAC
Suggested primer 2:GCTGATCTGGGAATCGCTAC