Insertion junction: LMJ.RY0402.141944_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.141944
Chromosome:chromosome 11
Location:2783496
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g477400 CYG43 (1 of 1) PF00072//PF00211//PF02518 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTGCCTTACCATTACCTGGCTACTGAG
Internal bar code:GTCAGAGTTTCGCTTTTGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:53
LEAP-Seq percent confirming:18.3673
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCGTTGGTACTGCCCAT
Suggested primer 2:TCCGGACTTACCATCCTGTC