Insertion junction: LMJ.RY0402.141944_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141944
Chromosome:chromosome 11
Location:2783505
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g477400 CYG43 (1 of 1) PF00072//PF00211//PF02518 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCGGTAAGAGCAGCGAGCGGCAGGCAC
Internal bar code:TCTTGCGCGCCAGCTCCTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:120
LEAP-Seq percent confirming:99.5575
LEAP-Seq n confirming:900
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCGTTGGTACTGCCCAT
Suggested primer 2:TCCGGACTTACCATCCTGTC