| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141958 |
| Chromosome: | chromosome 16 |
| Location: | 959556 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648750 | (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGCTCCTGCAGCCTCGTTGAAACCCT |
| Internal bar code: | CTCGAACGGCTCGACGAGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.9251 |
| LEAP-Seq n confirming: | 5336 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGAACAAACTGCATCCC |
| Suggested primer 2: | ACCAGGTCGCATCGTATAGC |