| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141974 |
| Chromosome: | chromosome 3 |
| Location: | 8754649 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205249 | (1 of 1) PTHR24115//PTHR24115:SF451 - FAMILY NOT NAMED // KINESIN-LIKE PROTEIN SUBITO | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGCGAGGTGGACGAGGAGGCCGCGGGG |
| Internal bar code: | ATTCGACACGTGTAATGAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 1493 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCACATGTAACGTGCCT |
| Suggested primer 2: | CCTTGTGGTGTGTGTGTGTG |