| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141974 |
| Chromosome: | chromosome 3 |
| Location: | 8756345 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205249 | (1 of 1) PTHR24115//PTHR24115:SF451 - FAMILY NOT NAMED // KINESIN-LIKE PROTEIN SUBITO | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGCGCATGTTCGCCACAGGAGCGGA |
| Internal bar code: | GGGGAGATTCGGAAAAGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 19.3042 |
| LEAP-Seq n confirming: | 688 |
| LEAP-Seq n nonconfirming: | 2876 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAAGGCCCACATCTGAC |
| Suggested primer 2: | AGAATGTGGGCTCCAAACTG |