| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142014 |
| Chromosome: | chromosome 3 |
| Location: | 3486701 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167600 | CaM-IP3,FAP61 | (1 of 1) PF16092 - Domain of unknown function (DUF4821) (DUF4821); Flagellar Associated Protein 61 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGCGGCGGGCTGCGGCTCGTCTGGTC |
| Internal bar code: | GCCTGCTAGTTTGGAACGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.8959 |
| LEAP-Seq n confirming: | 1920 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAACCCACTCGAACAAG |
| Suggested primer 2: | TGGTGACAGAACTGGAGCTG |