| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142017 |
| Chromosome: | chromosome 13 |
| Location: | 1678287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574050 | (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGGTGCGGTGCATCCCACGGGCTGC |
| Internal bar code: | TGGACGGGTGGATCTGTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.6922 |
| LEAP-Seq n confirming: | 2915 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGTGATGCAGCACTCC |
| Suggested primer 2: | ATCCTTGCCTCTTTTCGGAT |