| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142049 |
| Chromosome: | chromosome 13 |
| Location: | 2202416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578451 | TSL1 | (1 of 2) 6.1.1.4 - Leucine--tRNA ligase / Leucyl-tRNA synthetase; Leucyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGTGAAATAATACGAGAGGGCGTAGC |
| Internal bar code: | CATTCTAGCAATTAAAGTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 99.512 |
| LEAP-Seq n confirming: | 16926 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATCTTGTCCCTCTCCAA |
| Suggested primer 2: | AACATTCGTGCAGCCTTACC |