| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142065 |
| Chromosome: | chromosome 2 |
| Location: | 2844441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGAGTTCCGACACGTTATGTCAACTTG |
| Internal bar code: | CGCATATGCCATCACCAAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 99.1135 |
| LEAP-Seq n confirming: | 2795 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGACGACATGTAAGGGC |
| Suggested primer 2: | GTTGATCGAATCCTTGCCAT |