| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142075 |
| Chromosome: | chromosome 1 |
| Location: | 227140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001450 | Predicted protein; (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTTCGTGCGCGGCAGGCGGACATCTG |
| Internal bar code: | AGTCCAAGTGTCGGCGAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 95.591 |
| LEAP-Seq n confirming: | 33345 |
| LEAP-Seq n nonconfirming: | 1538 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCCTCTTGCTCGGCTG |
| Suggested primer 2: | AAATACGTGCAGGTAACGGG |