| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142076 |
| Chromosome: | chromosome 9 |
| Location: | 496443 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404201 | PHC30,PHC24,PHC10 | Pherophorin-chlamydomonas homolog 30; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCCATCTGTGGGTGCACCTAGACGCAG |
| Internal bar code: | CGTGTCGAGCCGATGGAGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 40.3859 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 1761 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACCATGTATGTAGCGG |
| Suggested primer 2: | GTGTTCGAGTTGTTTCGGGT |