| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142090 |
| Chromosome: | chromosome 2 |
| Location: | 5725196 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110200 | THO1 | Oligoendopeptidase; (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAATGAAATGTGGCTTGGTGGTGCGCC |
| Internal bar code: | AAGACCGTTCCTAGTGTTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 99.4391 |
| LEAP-Seq n confirming: | 1950 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGTGTTGCGGACTTACC |
| Suggested primer 2: | GGGCATGGGAGTAAAAGTCA |