| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142157 |
| Chromosome: | chromosome 13 |
| Location: | 869165 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567450 | HON1 | Histone H1; (1 of 3) K11275 - histone H1/5 (H1_5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGGGCTTGAGGTGAGGTATGCCATCGC |
| Internal bar code: | ATATACCTTCCGCCTTTTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 99.4739 |
| LEAP-Seq n confirming: | 6050 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTCCTCTGTGGAAGGT |
| Suggested primer 2: | GTGTGCAAATCAACCCTGTG |