| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142213 |
| Chromosome: | chromosome 10 |
| Location: | 773176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTCTGAGGAAGGCACACAAATCGTAG |
| Internal bar code: | GGAAATCACATAGGTTGTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 90.4722 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 230 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTTGCCAAGCAGCATGA |
| Suggested primer 2: | TGGGAAATCCAAGAAGTTGC |