Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.142236 |
Chromosome: | chromosome_1 |
Location: | 7945110 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g065822 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACCACAGTAGCACCTTGGCTGCACTAGCG |
Internal bar code: | GCTTTGCTCCCGGAGGCTGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 806 |
LEAP-Seq percent confirming: | 99.5443 |
LEAP-Seq n confirming: | 2840 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTTCATGCTGCTTGTGGTC |
Suggested primer 2: | ATCGAGTCGAATCGCACTTT |