| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.142236 |
| Chromosome: | chromosome 1 |
| Location: | 7945110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g065822 | FAP80,IFT122 | (1 of 54) IPR001680//IPR017986 - WD40 repeat // WD40-repeat-containing domain; Intraflagellar Transport Protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACAGTAGCACCTTGGCTGCACTAGCG |
| Internal bar code: | GCTTTGCTCCCGGAGGCTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.5443 |
| LEAP-Seq n confirming: | 2840 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCATGCTGCTTGTGGTC |
| Suggested primer 2: | ATCGAGTCGAATCGCACTTT |