| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.142241 |
| Chromosome: | chromosome 9 |
| Location: | 4973730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397919 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCATTCACACCGCAGGACAGCGGTCA |
| Internal bar code: | CTTCGGAGGGGTTGCGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 90.9275 |
| LEAP-Seq n confirming: | 1794 |
| LEAP-Seq n nonconfirming: | 179 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACACACACTCACACTTG |
| Suggested primer 2: | CATCCTGAAGGTGGTGAGGT |