| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.142313 |
| Chromosome: | chromosome 13 |
| Location: | 4897636 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605900 | OPR128 | OctotricoPeptide Repeat protein 128 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGGGTTGGTGGCGGCTGGCCAGCTGGA |
| Internal bar code: | GTGATAAACGGCGTGGAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 98.7603 |
| LEAP-Seq n confirming: | 239 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCTTACTAGTCGCCGGT |
| Suggested primer 2: | ACCTGACCTCCTGTCCAATG |