Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.142314 |
Chromosome: | chromosome 12 |
Location: | 2035583 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g509950 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTATGTGTACCCCTTCTTCTATGTGTCC |
Internal bar code: | CTATGAAGCGCTCAGTGTCGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 851 |
LEAP-Seq percent confirming: | 99.6309 |
LEAP-Seq n confirming: | 3239 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTACTGCTCGGATTTTGC |
Suggested primer 2: | TGGCTACGCCTTCACTTCTT |