Insertion junction: LMJ.RY0402.142344_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142344
Chromosome:chromosome 10
Location:2396615
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGACAGTTGCTTATGCAGAGCTGCCTT
Internal bar code:AGGGGCCGACAGTCCAGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:578
LEAP-Seq percent confirming:99.3023
LEAP-Seq n confirming:427
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTGCTACACAGAACCA
Suggested primer 2:TGCAACGCTATGGAGCTATG