Insertion junction: LMJ.RY0402.142349_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142349
Chromosome:chromosome 6
Location:2161945
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g265900 (1 of 1) K07023 - putative hydrolases of HD superfamily (K07023) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCATGATTCACAGCAGTGCGCCTCAAG
Internal bar code:GTGATGCATCCGGGTAACGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:657
LEAP-Seq percent confirming:62.2368
LEAP-Seq n confirming:1803
LEAP-Seq n nonconfirming:1094
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATCTGTGACCATTGTTG
Suggested primer 2:TCATTAACACGCAGGCTCAG