| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.142375 |
| Chromosome: | chromosome 11 |
| Location: | 709139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467627 | (1 of 1) 1.14.13.134 - Beta-amyrin 11-oxidase / CYP88D6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGCAGTTGGGGCATCTGGGCGGGCTTC |
| Internal bar code: | ACGCATCGGGCAGACATGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 99.8643 |
| LEAP-Seq n confirming: | 736 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCGCGTTCCTGACAACT |
| Suggested primer 2: | AGGTCGTGTGTGTGTGTGGT |